120 Ridge View Drive
Dunmore, PA 18512
Biography
Discipline Focus: Research in ecology of freshwater plankton, chrysophyte algae, mixotrophy, stomatocysts.
MEMBERSHIPS
- The American Society of Limnology and Oceanography
- British Society for Protist Biology / Protistology UK
- Northeast Algal Society
- Member of the Penn State Scranton Speakers' Bureau.
Research Interests
Dr. Holen studies lake ecology. He is interested in the ecology of mixotrophic algae and stomatocyst production in golden-brown (chrysophyte) algae.
Research interests:
- Limnology
- Algae
- Protists
- Mixotrophy
- Stomatocysts
- Chrysophytes
Publications
- Hamsher S. E., Ellis K., Holen D., Sanders. R. W. (2020) Effects of light, dissolved nutrients on ingestion and growth of a newly identified mixotrophic alga Chrysoplepidomonas dendrolepidota (Chrysophyceae). Hydrobiol. 847: 2923 – 2932.
- Holen, D.A. (2014) Chrysophyte stomatocyst production in laboratory culture and descriptions of seven cyst morphotypes. Phycol. 53 (5): 426-432.
- Holen, D.A. (2010) Mixotrophy in two species of Ochromonas. Nova Hedwigia, Beiheft 136, 153-165.
- Holen, D.A. (2000) The effects of heterotrophy on Chlorophyll a and photosynthesis in a mixotrophic chrysophyte. Nova Hedwigia. 122: 107-118.
- Holen D.A. (2000) The relative abundance of mixotrophic and heterotrophic ciliates in an oligotrophic lake. Archiv Hydrobiol. 150: 1-15.
- Holen, D.A. (1999) Effects of prey abundance and light intensity on the mixotrophic chrysophyte Poterioochromonas malhamensis from a mesotrophic Lake. Fresh. Biol. 42: 445-455.
- Wehr, J.D., D.A. Holen, M.M. MacDonald and S.P. Lonegran. 1998. Effects of different organic carbon sources on a freshwater plankton community. Can. J. Fish. Aquat Sci. 55: 2150-2160.
- Boxhorn, J.E., D.A. Holen and M.E. Boraas. 1998. Toxicity of the Chrysophyte flagellate Poterioochromonas malhamensis to the rotifer Brachionus angularis. Hydrobiol. 387/388: 283-287.
- Holen, D.A. and M.E. Boraas. 1995. Mixotrophy in Chrysophytes. In: Chrysophyte Algae: Ecology, Physiology and Development. C. Sandgren, J Smol and Kristiansen, eds. Cambridge Univ. Press.
- Holen, D.A. and M.E. Boraas. 1991. The feeding behavior of Spumella sp. as a function of particle size: implications for bacterial size in pelagic environments. Hydrobiol. 220: 73-88.
- Gillott, M.A., D.A. Holen, J. Ekman, and M. Boraas. 1993. Predation induced E. coli filaments: Are they multicellular? In: Proc. 51st Annual meeting of the Microscopy Society of America. G.W. Bailey and C.L. Reider, eds. San Francisco Press.
- Boraas, M.E., D.W. Bolgrien and D.A. Holen. 1991. Determination of eubacterial and cyanobacterial size and number in Lake Baikol using epifluorescence. Internationale Revue Gesmaten Hydrobiol. 76(4): 537-544.
- Boraas, M.E., D.M. Seale, and D.A. Holen. 1991. Predatory behavior of Ochromonas analyzed with video microscopy. Arch. fur Hydrobiol. 123(4): 459-468.
- Seale, D.M., M.E. Boraas, D.A. Holen and K. Nealson. 1990. Use of bioluminescent bacteria, Xenorhabdus luminescens, to measure predation by a freshwater microflagellate. FEMS Microbiology. 73: 31-40.
Education
B.S., Water Resource Management, University of Wisconsin - Stevens Point, 1976
M.S., Biological Sciences, University of Wisconsin-Milwaukee,
Thesis Title: The feeding behavior of Spumella sp. as a function of particle size: implications for bacterial size in pelagic systems. 1988
Ph.D., Biological Sciences, University of Wisconsin-Milwaukee,
Thesis Title:Physiological studies of mixotrophy in the algal flagellate Poterioochromonas malhamensis (Chrysophyte) using batch and continuous cultures. 1994
Papers and Presentations
PRESENTATIONS
- 2019 Stomatocyst morphotype and encystment rates in Ochromonas pinguis: A mixotrophic Chrysophyte. Northeast Ecology Conference, Edgedale, PA
- 2019 The distribution of Prorodon viridis (Ciliphora, Prostomatida), A ciliate with endosymbiotic Chlorella (Trebouxiophyceae, Chorellales), at the oxic/anoxic interface in a freshwater lake. Northeast Algal Society Symposium, Salem, MA.
- 2019 Stomatocyst morphotypes and Induction Rates in Ochromonas pinguis. Association for the sciences of limnology and oceanography, San Juan, Puerto Rico.
- 2018 Stomatocyst production and environmental inducement in Ochromonas pinguis, a mixotrophic Chrysophyte. Northeast Algal Society Symposium, University of New Haven, CT
- 2015 Chrysophyte stomatocyst production in laboratory culture and descriptions of seven cyst morphotypes. Northeast Algal Society, Syracuse, NY.
- 2013 Mixotrophy in Chrysolepidomonas dendrolepidota, a chrysophycean alga, Lacawac Ecology Conference, Lake Ariel.
- 2013 Phagotrophy in the scaled chrysophyte Chrysolepidomonas dendrolepidota, Northeast Algal Society, Mystic, Ct.
- 2012 The Stomatocysts of Ochromonas sp., (Chrysophyceae) A Small Mixotrophic Alga, Northeast Algal Society, Schoodic Point, Maine.
- 2011 Encystment by the mixotrophic flagellate Ochromonas pinguis (Chrysophyceae) in laboratory culture., Northeast Algal Society, Woods Hole, MA.
- 2010 The Stomatocyst of Ochromonas pinguis Conrad, 49th Northeast Algal Symposium, Roger Williams University, Bristol RI.
- 2010 Mixotrophy in Two Species of Ochromonas, UP Forestry Department, University Park.
- 2008 Mixotrophy in two species of Ochromonas, British Society for Protist Biology, Gregynog, Wales,